Tested Applications
| Positive WB detected in | RAW 264.7 cells, Recombinant protein, mouse skin tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85876-1-RR targets MGL2 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2727 Product name: Recombinant Mouse MGL2 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 72-332 aa of NM_145137.2 Sequence: SQNSQLRRDLGTLRAILDNTTSKIKAEFQSLDSRADNFEKGISSLKVDVEDHRQELQAGRDLSQKVTSLESTLEKREQALKTDLSDLTDHVQQLETDLKALTCQLANLKNNGSEVACCPLHWTEHEGSCYWFSESEKSWPEADKYCRLENSHLVVVNSLEEQNFLQNRLANVLSWMGLTDQNGPWRWVDGTDFDKGFKNWRPLQPDNWHGHMLGGGEDCAHFSYDGRWNDDVCQRHYHWICETELGKASSAHSPQLIASVP Predict reactive species |
| Full Name | macrophage galactose N-acetyl-galactosamine specific lectin 2 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | NM_145137.2 |
| Gene Symbol | Mgl2 |
| Gene ID (NCBI) | 216864 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8JZN1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MGL2 is mainly expressed on immature dendritic cell (DCs) , tolerogenic DCs, type 2 DCs, and alternatively activated macrophages. MGL2 protein has carbohydrate-binding activity, specifically recognizing and binding to terminal GalNAc (n-acetyl 2-amino-2-deoxy-D-galactopyranose) residues, including the TN antigen (Galnac- αthrser) . This specific binding makes it play an important role in the recognition and processing of glycosylated pathogens, apoptotic cell fragments and tumor cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MGL2 antibody 85876-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
