FC experiment of mouse bone marrow using 98269-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD45 (30-F11) (APC-65087, Clone: 30-F11). Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD45 (30-F11) (APC-65087, Clone: 30-F11). Cells were not fixed.
FC experiment of mouse bone marrow using 98269-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
Affinity and Kinetic Characterization of 98269-1-RR
Biolayer interferometry (BLl) kinetic assays of 98269-1-RR against Mouse Glycophorin A/CD235a were performed. The affinity constant is 19.4 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98269-1-RR targets Glycophorin A/CD235a in FC applications and shows reactivity with mouse samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)
Protocols
Product Specific Protocols
FC protocol for Glycophorin A/CD235a antibody 98269-1-RR
FC experiment of mouse bone marrow using 98269-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD45 (30-F11) (APC-65087, Clone: 30-F11). Cells were not fixed.
FC experiment of mouse bone marrow using 98269-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98269-1-RR
Biolayer interferometry (BLl) kinetic assays of 98269-1-RR against Mouse Glycophorin A/CD235a were performed. The affinity constant is 19.4 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
Glycophorin A/CD235a Recombinant antibody
Catalog Number
98269-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
