Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody, PBS Only

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  FC experiment of mouse bone marrow using 98269-1-RR (same clone as 98269-1-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD45 (30-F11) (APC-65087, Clone: 30-F11). Cells were not fixed. This data was developed using the same antibody clone with 98269-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse bone marrow using 98269-1-RR (same clone as 98269-1-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98269-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98269-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98269-1-RR against Mouse Glycophorin A/CD235a were performed. The affinity constant is 19.4 nM.

Glycophorin A/CD235a {ptg:Brand} Recombinant Antibody for FC

Cat No. 98269-1-PBS

Clone No.242129D10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

glycophorin A, Gypa, GPA, Glycophorin-A, CD235a

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Product Information

98269-1-PBS targets Glycophorin A/CD235a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: glycophorin A
• Calculated Molecular Weight: 18kDa
• GenBank Accession Number: NM_010369.3
• Gene Symbol: Gypa
• Gene ID (NCBI): 14934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P14220
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)