Tested Applications
| Positive FC detected in | Transfected CHO |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98582-1-RR targets TNFSF12 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2870 Product name: Recombinant Human TNFSF12 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 93-249 aa of NM_003809.2 Sequence: RSAPKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTYFGLFQVH Predict reactive species |
| Full Name | tumor necrosis factor (ligand) superfamily, member 12 |
| Calculated Molecular Weight | 27 kDa |
| GenBank Accession Number | NM_003809.2 |
| Gene Symbol | TNFSF12 |
| Gene ID (NCBI) | 8742 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O43508-1 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
TNFSF12, also known as TWEAK (TNF-like weak inducer of apoptosis), is a member of the tumor necrosis factor ligand superfamily. It functions as a cytokine by binding to its primary receptor, Fn14 (TNFRSF12A), and plays diverse roles in immune regulation, angiogenesis, tissue repair, and inflammation (PMID: 39905000). TNFSF12 is upregulated in multiple solid tumors and promotes cancer cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) (PMID: 28639899).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TNFSF12 antibody 98582-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
