Recombinant Human TNFSF11/RANKL protein (His Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0982
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | CHO-derived Human TNFSF11 protein Gly64-Asp245 (Accession# AAC51762.1) with a His tag at the N-terminus. |
| GeneID | 8600 |
| Accession | AAC51762.1 |
| PredictedSize | 21.3 kDa |
| SDS-PAGE | 22-27 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is produced by a diverse set of lineage-specific cells and is involved in a wide variety of physiological processes that include skeletal remodeling, lymph node organogenesis, mammary gland development, and thermal regulation. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy.
References:
1.GiulianiN.etal.(2004)ExpHematol.32(8):685-91. 2.HanadaR.etal.(2011)JMolMed(Berl).89(7):647-56. 3.OnalM.etal.(2015)JBoneMinerRes.30(5):855-68. 4.DanksL.etal.(2016)AnnRheumDis.75(6):1187-95.