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Recombinant Mouse Tissue Factor protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1102

Validation Data Gallery

  • Purity of Recombinant Mouse Tissue Factor was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Tissue Factor was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Tissue Factor protein Ala29-Glu251 (Accession# P20352) with a His tag at the C-terminus.
GeneID 14066
Accession P20352
PredictedSize 26.5 kDa
SDS-PAGE 30-40 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Tissue factor (TF),also named coagulation factor III or F3, is a transmembrane protein that functions as a high-affinity receptor for factor (F)VII and FVIIa. The TF-FVIIa complex is the primary initiator of blood coagulation and plays an essential role in hemostasis. TF is expressed on perivascular cells and epithelial cells at organ and body surfaces where it forms a hemostatic barrier. TF also provides additional hemostatic protection to vital organs, such as the brain, lung, and heart. Under pathological conditions, TF can trigger both arterial and venous thrombosis.

References:

1. Grover SP. et al. (2018). Arterioscler Thromb Vasc Biol. 38(4):709-725.
2. Mackman N. et al.(2007). Arterioscler Thromb Vasc Biol. 27:1687–1693.
3. Rao LV. et al. (2012). J Thromb Haemost. 10:2242–2253.
4. Witkowski M. et al. (2016). Trends Cardiovasc Med. 26(4):297-303.