Recombinant Human Siglec-5/CD170 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg1673
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Siglec-5 protein Glu17-Thr434 (Accession# O15389) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 8778 |
| Accession | O15389 |
| PredictedSize | 72.6 kDa |
| SDS-PAGE | 85-110 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
SIGLEC5, also known as sialic acid-binding immunoglobulin-like lectin 5, is a member of the Siglec family, which are a group of immunoglobulin-type lectins that mediate protein-carbohydrate interactions via sialic acids attached to glycoproteins or glycolipids. SIGLEC5 is part of the CD33-related subset of Siglecs (CD33rSiglecs) and inhibits the activation of several cell types, including monocytes, macrophages, and neutrophils. In addition, SIGLEC5 mediates sialic acid-dependent binding to human erythrocytes and soluble glycoconjugates, indicating its potential involvement in cell-cell interactions.
References:
1. Vuchkovska A. et al. (2022). Immunology. 166(2):238-248. 2. Cornish AL. et al. (1998). Blood. 1998;92(6):2123-2132.