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Recombinant Human CD83 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2033

Validation Data Gallery

  • Purity of Recombinant Human CD83 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD83 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD83 protein Thr20-Ala143 (Accession# Q01151) with a rabbit IgG Fc tag at the C-terminus.
GeneID 9308
Accession Q01151
PredictedSize 40.3 kDa
SDS-PAGE 42-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils. CD83 regulates the maturation, activation, and homeostasis of numerous immune cells. CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation.

References:

1. Alexander T Prechtel, Alexander Steinkasserer. (2007) Arch Dermatol Res. May;299(2):59-69.
2. Ziduo Li, Xinsheng Ju, et al. (2019) Front Immunol. Jun 7:10:1312.
3. Linda Grosche, Ilka Knippertz, et al. (2020) Front Immunol.  Apr 17:11:721.
4. Matthias Lechmann, Elisabeth Zinser, et al. (2002) Int Arch Allergy Immunol. Oct;129(2):113-8.