Recombinant Human Integrin alpha-1 beta-1 Heterodimer protein (rFc Tag & His Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag & His Tag
Activity
not tested
Cat no : Eg2089
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Integrin alpha-1 beta-1 heterodimer protein, Human Integrin alpha-1 protein Phe29-Pro1141 (Accession# P56199) with a rabbit IgG Fc tag at the C-terminus, Human Integrin beta-1 protein Gln21-Asp728 (Accession# P05556-1) with a his tag at the C-terminus. |
| GeneID | 3672 and 3688 |
| Accession | P56199 and P05556-1 |
| PredictedSize | 149.4 kDa (Integrin alpha-1) and 79.4 kDa (Integrin beta-1) |
| SDS-PAGE | 150-220 kDa (Integrin alpha-1) and 90-120 kDa(Integrin beta-1), reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands. They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits. In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis. Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa.
References:
1. Takada, Yoshikazu et al. Genome biology vol. 8,5 (2007): 215. 2. Harburger, David S, and David A Calderwood. Journal of cell science vol. 122,Pt 2 (2009): 159-63.