Recombinant Human ADAMTS13 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2556
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | CHO-derived Human ADAMTS13 protein Gln34-Trp688 (Accession# Q76LX8-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 11093 |
| Accession | Q76LX8-1 |
| PredictedSize | 97.4 kDa |
| SDS-PAGE | 28-30 kDa and 90-115 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motifs, member 13) is a zinc-dependent metalloproteinase that contains multiple domains, such as the signal peptide, propeptide, metalloproteinase domain, disintegrin-like domain, and TSP-1 domain, among others. The primary function of ADAMTS13 is to specifically cleave the ultra-large multimers of von Willebrand factor (vWF), preventing excessive platelet aggregation and the formation of microvascular thrombi. Deficiency of ADAMTS13 is closely associated with thrombotic thrombocytopenic purpura (TTP), a severe disease characterized by thrombocytopenia, microvascular thrombosis, and tissue damage. Additionally, reduced ADAMTS13 activity is also linked to diseases such as myocardial infarction and stroke.
References:
1. Papakonstantinou, Anna et al. International journal of molecular sciences vol. 25,15 8137. 26 Jul. 2024. 2. DeYoung, Veronica et al. Journal of thrombosis and haemostasis : JTH vol. 20,12 (2022): 2722-2732. 3. Plautz, William E et al. Transfusion vol. 58,10 (2018): 2453-2462.