Recombinant Human CD244 protein (Myc Tag, His Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
Myc Tag, His Tag
Activity
not tested
Cat no : Eg32087
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CD244 protein Cys22-Arg221 (Accession# Q9BZW8-2) with a Myc tag and a His tag at the C-terminus. |
| GeneID | 51744 |
| Accession | Q9BZW8-2 |
| PredictedSize | 27.9 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs. CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function.
References:
1. Brown, M H et al. The Journal of experimental medicine vol. 188,11 (1998): 2083-90. 2. Agresta, Laura et al. Frontiers in immunology vol. 28,9 (2018) 2809. 3. Chlewicki, Lukasz K et al. Journal of immunology (Baltimore, Md. : 1950) vol. 180,12 (2008): 8159-67.