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Recombinant Human CD58 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg4236

Validation Data Gallery

  • Purity of Recombinant Human CD58 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD58 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human CD58 was greater than 90% as determined by SEC-HPLC.

    The purity of Human CD58 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD58 protein Phe29-Arg215 (Accession# Q9BRW0) with a rabbit IgG Fc tag at the C-terminus.
GeneID 965
Accession Q9BRW0
PredictedSize 47.7 kDa
SDS-PAGE 55-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD58, also known as LFA-3 (Lymphocyte Function-Associated Antigen 3), is a cell surface glycoprotein that plays a crucial role in immune cell interactions. It is a member of the immunoglobulin superfamily and acts as a ligand for CD2, primarily expressed in T and NK cells. The CD2-CD58 interaction is an important component of the immunological synapse that induces activation and proliferation of T/NK cells, triggering a series of intracellular signaling pathways. CD58 has been implicated in various diseases, particularly in the context of cancer. It acts as a tumor promotor in hepatocellular carcinoma via activating the AKT/GSK-3β/β-catenin pathway.

References:

1.Wang C. et al. (2023). J Transl Med. 21(1):539.
2.Zhang Y. et al. (2021). Front Immunol. 12:705260. 
3.Wu B. et al. (2024). Commun Biol. 7(1):1025.