Recombinant Mouse IGSF8/CD316 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg6320
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse IGSF8 protein Arg26-Thr577 (Accession# Q8R366) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 140559 |
| Accession | Q8R366 |
| PredictedSize | 84.3 kDa |
| SDS-PAGE | 84-115 kDa , reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
IGSF8 (CD316/EWI‑2) is a type I transmembrane glycoprotein of immunoglobulin superfamily, interacting tightly with tetraspanins CD9 and CD81 to modulate membrane trafficking and cell adhesion. Widely expressed on immune and neuronal cells, it functions as a novel innate immune checkpoint: overexpressed in diverse malignancies, it binds NK‑cell KIR3DL2 to block NK‑mediated tumor clearance. Preclinical and early clinical data validate it as a promising immunotherapy target; blocking anti‑IGSF8 mAbs boost antitumor immunity alone or combined with anti‑PD1 regimens.
References:
1. Li, Yulong et al. Cell vol. 187,11 (2024): 2703-2716.e23.