4-Hydroxynonenal Monoclonal antibody

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  ELISA experiment of 4-Hydroxynonenal using 68538-1-Ig

  Indirect ELISA was performed by coating BSA conjugated 4-Hydroxynonenal (4-HNE) at ~20 ng/well (by 4-HNE amount), followed by blocking with 1% BSA. Serial diluted 4-Hydroxynonenal antibody 68538-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of 4-Hydroxynonenal was mixed in 5 ng/mL primary antibody (bottom X-left Y, red curve).

4-Hydroxynonenal Monoclonal Antibody for ELISA

Cat No. 68538-1-Ig

Clone No.2C1D10

Publications(14)

Host / Isotype

Mouse / IgG2a

Reactivity

4-hydroxynonenal, chemical compound and More (3)

Applications

WB, IHC, IF, ELISA

Synonyms

4-HNE, 4-Hydroxy-2-nonenal, C9H16O2, Cas. 75899-68-2

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Tested Applications

• Positive ELISA detected in: 4-Hydroxynonenal

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 7 publications below

Product Information

68538-1-Ig targets 4-Hydroxynonenal in WB, IHC, IF, ELISA applications and shows reactivity with 4-hydroxynonenal, chemical compound samples.

• Tested Reactivity: 4-hydroxynonenal, chemical compound
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: 4-Hydroxynonenal
• RRID: AB_3085246
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

4-Hydroxynonenal is a uremic toxin. Uremic toxins can be subdivided into three major groups based upon their chemical and physical characteristics: 1) small, water-soluble, non-protein-bound compounds, such as urea; 2) small, lipid-soluble and/or protein-bound compounds, such as the phenols and 3) larger so-called middle-molecules, such as beta2-microglobulin. 4-Hydroxynonenal (4-HNE) is a major aldehydic product of ω-6-unsaturated fatty acid peroxidation. It is considered a lipid peroxidation specific marker. 4-HNE has been found to induce differentiation and inhibit proliferation of HL-60 human leukemic cells. It has also been found to induce murine alveolar macrophage cell death. 4-HNE has been shown to inhibit State 3 respiration, causing a transient cytosolic Ca2+ increase. In addition, it irreversibly inhibits Na+-K+-ATPase activity.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Free Radic Biol Med; Mitochondrial ACSS1-K635 acetylation knock-in mice exhibit altered liver lipid metabolism on a ketogenic diet; Authors - Guogang Xu
mouse	IF	Biomaterials; Molybdenum nanodots act as antioxidants for photothermal therapy osteoarthritis; Authors - Guang Shi
mouse	IHC	Redox Biol; Identification of Pinostilbene as a natural STING agonist that triggers FTH1 degradation via K48-ubiquitination to induce ferroptosis in non-small cell lung cancer.; Authors - Shuangshuang Song
mouse	IHC	J Immunother Cancer; Epigenetic suppression of Nrf2-Slc40a1 axis induces ferroptosis and enhances immunotherapy in pancreatic cancer.; Authors - Yixuan Zhang
human	IF	Bioact Mater; ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells.; Authors - Shang Chen
mouse	WB,IF	Redox Biol; A spinal USP7-Bach1 positive feedback loop drives NOX4-mediated ferroptosis in neuropathic pain.; Authors - Bo Jiao