Alpha 1B-Glycoprotein Monoclonal antibody

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  WB analysis of human plasma using 66260-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66260-1-Ig (Alpha 1B-Glycoprotein antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66260-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66260-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (Alpha 1B-Glycoprotein antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66260-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66260-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 66260-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Alpha 1B-Glycoprotein Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66260-1-Ig

Clone No.4D4E5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

A1BG, 4D4E5, A1B, ABG, alpha 1 B glycoprotein

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Tested Applications

• Positive WB detected in: human plasma, human heart tissue, human placenta tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66260-1-Ig targets Alpha 1B-Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5414

  Product name: Recombinant human A1BG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-373 aa of BC035719

  Sequence: MAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES

• Full Name: alpha-1-B glycoprotein
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC035719
• Gene Symbol: A1BG
• Gene ID (NCBI): 1
• RRID: AB_2881647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P04217
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).