CoraLite®594-conjugated Alpha 1B-Glycoprotein Monoclonal antibody

Alpha 1B-Glycoprotein Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4D4E5

Cat no : CL594-66260

Synonyms

A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, DKFZp686F0970, GAB, HYST2477

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL594-66260

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Alpha 1B-Glycoprotein antibody (CL594-66260, Clone: 4D4E5 ) at dilution of 1:100.

---

Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL594-66260 targets Alpha 1B-Glycoprotein in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha 1B-Glycoprotein fusion protein Ag5414
• Full Name: alpha-1-B glycoprotein
• Calculated molecular weight: 54 kDa
• Observed molecular weight: 72 kDa
• GenBank accession number: BC035719
• Gene symbol: A1BG
• Gene ID (NCBI): 1
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).