Filter: AllWBIHCIF/ICC

WB analysis of human plasma using 66260-1-Ig (same clone as 66260-1-PBS)
human plasma were subjected to SDS PAGE followed by western blot with 66260-1-Ig (Alpha 1B-Glycoprotein antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 66260-1-Ig (same clone as 66260-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 66260-1-Ig (same clone as 66260-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (Alpha 1B-Glycoprotein antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66260-1-Ig (same clone as 66260-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66260-1-Ig (same clone as 66260-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66260-1-Ig (same clone as 66260-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66260-1-PBS in a different storage buffer formulation.

Alpha 1B-Glycoprotein Monoclonal antibody, PBS Only

Alpha 1B-Glycoprotein Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66260-1-PBS

Clone No.4D4E5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

A1BG, 4D4E5, A1B, ABG, alpha 1 B glycoprotein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66260-1-PBS targets Alpha 1B-Glycoprotein in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5414 Product name: Recombinant human A1BG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-373 aa of BC035719 Sequence: MAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES Predict reactive species
Full Name	alpha-1-B glycoprotein
Calculated Molecular Weight	54 kDa
Observed Molecular Weight	72 kDa
GenBank Accession Number	BC035719
Gene Symbol	A1BG
Gene ID (NCBI)	1
RRID	AB_2881647
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P04217
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).