AACS Polyclonal antibody, PBS Only

• 

  WB analysis of mouse heart using 13815-1-AP (same clone as 13815-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 13815-1-AP (same clone as 13815-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 13815-1-AP (same clone as 13815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13815-1-AP (AACS antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 13815-1-AP (same clone as 13815-1-PBS)

  Immunofluorescent analysis of MCF-7 cells using 13815-1-AP (AACS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

AACS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13815-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1

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Product Information

13815-1-PBS targets AACS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4899

  Product name: Recombinant human AACS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 322-672 aa of BC040490

  Sequence: TSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF

• Full Name: acetoacetyl-CoA synthetase
• Calculated Molecular Weight: 672 aa, 75 kDa
• Observed Molecular Weight: 67 kDa, 30 kDa
• GenBank Accession Number: BC040490
• Gene Symbol: AACS
• Gene ID (NCBI): 65985
• RRID: AB_2877977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86V21
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.