CoraLite® Plus 488-conjugated ACADM Monoclonal antibody

ACADM Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A11G6

Cat no : CL488-67742

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-67742

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ACADM antibody (CL488-67742, Clone: 1A11G6 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67742 targets ACADM in IF-P applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACADM fusion protein Ag30620
• Full Name: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Calculated Molecular Weight: 421 aa, 47 kDa
• Observed Molecular Weight: 42-47 kDa
• GenBank Accession Number: BC005377
• Gene Symbol: ACADM
• Gene ID (NCBI): 34
• RRID: AB_2919535
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.