CoraLite® Plus 488-conjugated ACADM Monoclonal antibody

ACADM Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A11G6

Cat no : CL488-67742

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH



Tested Applications

Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67742 targets ACADM in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACADM fusion protein Ag30620
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Calculated Molecular Weight 421 aa, 47 kDa
Observed Molecular Weight42-47 kDa
GenBank Accession NumberBC005377
Gene Symbol ACADM
Gene ID (NCBI) 34
RRIDAB_2919535
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACADM antibody CL488-67742Download protocol
Standard Protocols
Click here to view our Standard Protocols