ACADVL Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85889-1-RR (same clone as 85889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85889-1-RR (ACADVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85889-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 85889-1-RR (same clone as 85889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACADVL antibody (85889-1-RR, Clone: 250199C12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85889-1-PBS in a different storage buffer formulation.

ACADVL Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85889-1-PBS

Clone No.250199C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

VLCAD, EC:1.3.8.9, Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

---

Product Information

85889-1-PBS targets ACADVL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6016

  Product name: Recombinant human ACADVL protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 312-655 aa of BC012912

  Sequence: FDGVRVPSENVLGEVGSGFKVAMHILNNGRFGMAAALAGTMRGIIAKAVDHATNRTQFGEKIHNFGLIQEKLARMVMLQYVTESMAYMVSANMDQGATDFQIEAAISKIFGSEAAWKVTDECIQIMGGMGFMKEPGVERVLRDLRIFRIFEGTNDILRLFVALQGCMDKGKELSGLGSALKNPFGNAGLLLGEAGKQLRRRAGLGSGLSLSGLVHPELSRSGELAVRALEQFATVVEAKLIKHKKGIVNEQFLLQRLADGAIDLYAMVVVLSRASRSLSEGHPTAQHEKMLCDTWCIEAAARIREGMAALQSDPWQQELYRNFKSISKALVERGGVVTSNPLGF

• Full Name: acyl-Coenzyme A dehydrogenase, very long chain
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC012912
• Gene Symbol: ACADVL
• Gene ID (NCBI): 37
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49748
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACADVL (Acyl-CoA Dehydrogenase, Very Long Chain) is a mitochondrial enzyme that catalyzes the initial step of β-oxidation for very long-chain fatty acids (VLCFAs, C14-C20). Deficiency of ACADVL causes very long-chain acyl-CoA dehydrogenase deficiency (VLCADD), the most common inherited disorder of mitochondrial fatty acid oxidation, characterized by hypoketotic hypoglycemia, cardiomyopathy, hepatopathy, and exercise-induced rhabdomyolysis. ACADVL is highly expressed in metabolically active tissues including cardiac muscle, skeletal muscle, and liver, where it plays a critical role in energy production during fasting and high-demand states. Mutations in ACADVL represent a significant diagnostic target for newborn screening and genetic counseling in metabolic disorders.