ACADVL Recombinant monoclonal antibody

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  WB analysis using 85889-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85889-1-RR (ACADVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 85889-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACADVL antibody (85889-1-RR, Clone: 250199C12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ACADVL Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85889-1-RR

Clone No.250199C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

VLCAD, EC:1.3.8.9, Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293T cells, A431 cells, L02 cells, mouse liver tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85889-1-RR targets ACADVL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6016

  Product name: Recombinant human ACADVL protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 312-655 aa of BC012912

  Sequence: FDGVRVPSENVLGEVGSGFKVAMHILNNGRFGMAAALAGTMRGIIAKAVDHATNRTQFGEKIHNFGLIQEKLARMVMLQYVTESMAYMVSANMDQGATDFQIEAAISKIFGSEAAWKVTDECIQIMGGMGFMKEPGVERVLRDLRIFRIFEGTNDILRLFVALQGCMDKGKELSGLGSALKNPFGNAGLLLGEAGKQLRRRAGLGSGLSLSGLVHPELSRSGELAVRALEQFATVVEAKLIKHKKGIVNEQFLLQRLADGAIDLYAMVVVLSRASRSLSEGHPTAQHEKMLCDTWCIEAAARIREGMAALQSDPWQQELYRNFKSISKALVERGGVVTSNPLGF

• Full Name: acyl-Coenzyme A dehydrogenase, very long chain
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC012912
• Gene Symbol: ACADVL
• Gene ID (NCBI): 37
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49748
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.