ACSL3 Polyclonal antibody, PBS Only

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  WB analysis of HEK-293 using 20710-1-AP (same clone as 20710-1-PBS)

  WB result of ACSL3 antibody (20710-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL3 transfected HEK-293 cells. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 20710-1-AP (same clone as 20710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20710-1-AP (ACSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ACSL3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 20710-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ACS3, ACSL 3, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.2

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Product Information

20710-1-PBS targets ACSL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: acyl-CoA synthetase long-chain family member 3
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_004457
• Gene Symbol: ACSL3
• Gene ID (NCBI): 2181
• RRID: AB_10697670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95573
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.