ACSL4/FACL4 Monoclonal antibody, PBS Only

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  WB analysis using 66617-1-Ig (same clone as 66617-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

  WB result of ACSL4 antibody (66617-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66617-1-Ig (same clone as 66617-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 66617-1-Ig (same clone as 66617-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66617-1-Ig (same clone as 66617-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66617-1-Ig (same clone as 66617-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 66617-1-Ig (same clone as 66617-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human ACSL4 (66617-1-Ig, Clone:1H5D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ACSL4/FACL4 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 66617-1-PBS

Clone No.1H5D3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

ACSL4, FACL4, 1H5D3, ACS4, ACSL 4

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Product Information

66617-1-PBS targets ACSL4/FACL4 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18085

  Product name: Recombinant human ACSL4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 143-236 aa of BC034959

  Sequence: LGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMY

• Full Name: acyl-CoA synthetase long-chain family member 4
• Calculated Molecular Weight: 711 aa, 79 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC034959
• Gene Symbol: ACSL4
• Gene ID (NCBI): 2182
• RRID: AB_2881977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60488
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.