ACTN3 Polyclonal antibody

ACTN3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 24378-1-AP

Synonyms

actinin, alpha 3, ACTN3, Alpha actinin 3, F actin cross linking protein



Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24378-1-AP is ACTN3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN3 fusion protein Ag19479
Full Name actinin, alpha 3
Calculated molecular weight 901 aa, 103 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC099647
Gene symbol ACTN3
Gene ID (NCBI) 89
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACTN3 antibody 24378-1-APDownload protocol
IHC protocol for ACTN3 antibody 24378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Dokl Biochem Biophys

Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Authors - I O Petrova

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-12-2019)

The antibody recognizes ACTN3 (top band) but also other non-specific proteins, though less prominently.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ACTN3 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:24378-1-AP)