|Positive WB detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||mouse skeletal muscle tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24378-1-AP is ACTN3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACTN3 fusion protein Ag19479|
|Full Name||actinin, alpha 3|
|Calculated molecular weight||901 aa, 103 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC099647|
|Gene ID (NCBI)||89|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-12-2019)
The antibody recognizes ACTN3 (top band) but also other non-specific proteins, though less prominently.