ADAMDEC1 Polyclonal antibody

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  WB analysis of MCF-7 using 17899-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 17899-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17899-1-AP (ADAMDEC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ADAMDEC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17899-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.4.24.-, ADAM-like protein decysin-1, ADAMDEC 1, ADAM DEC1, A disintegrin and metalloproteinase domain-like protein decysin-1

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

17899-1-AP targets ADAMDEC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAMDEC1 fusion protein Ag12265
• Full Name: ADAM-like, decysin 1
• Calculated Molecular Weight: 470 aa, 53 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC074877
• Gene Symbol: ADAMDEC1
• Gene ID (NCBI): 27299
• RRID: AB_2878461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15204
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Metalloendopeptidase ADAM-Like Decysin 1 (ADAMDEC1) was first identified as a unique member of the matrix metalloprotease (ADAM) family. ADAMs are increasingly recognized as playing an important role in gut homeostasis and bowel inflammation. ADAMDEC1 is abundantly, and almost exclusively, expressed in the gastrointestinal (GI) tract.

Publications

Species	Application	Title
human	IHC	Arch Toxicol; A transcriptome-wide association study integrating multi-omics bioinformatics and Mendelian randomization reveals the prognostic value of ADAMDEC1 in colon cancer; Authors - Cong Zhang
human	IHC	J Inflamm Res; The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome; Authors - Haishi Zheng
human,mouse	WB,IHC	Thorac Cancer; Upregulation of ADAMDEC1 correlates with tumor progression and predicts poor prognosis in non-small cell lung cancer (NSCLC) via the PI3K/AKT pathway.; Authors - Weiliang Zhu
human	IHC	Aging (Albany NY); An immune signature to predict the prognosis of ATRX-wildtype glioma patients and guide immune checkpoint blockade therapy; Authors - Wenpeng Cao
human	WB	Sci Rep; ADAMDEC1 promotes the malignant progression of cholangiocarcinoma by regulating NF-κB signaling pathway; Authors - Shuo Zhou
human	WB,IHC	Exp Cell Res; ADAMDEC1 induces EMT and promotes colorectal cancer cells metastasis by enhancing Wnt/β-catenin signaling via negative modulation of GSK3β; Authors - Yuna Jia ; KD Validated