ADAMDEC1 Polyclonal antibody

ADAMDEC1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17899-1-AP

Synonyms

ADAM DEC1, ADAM like protein decysin 1, ADAM like, decysin 1, ADAMDEC1, M12.219

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Validation Data Gallery

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  WB analysis of MCF-7 using 17899-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 17899-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17899-1-AP (ADAMDEC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 17899-1-AP is ADAMDEC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAMDEC1 fusion protein Ag12265
• Full Name: ADAM-like, decysin 1
• Calculated molecular weight: 470 aa, 53 kDa
• Observed molecular weight: 44 kDa
• GenBank accession number: BC074877
• Gene symbol: ADAMDEC1
• Gene ID (NCBI): 27299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human,mouse	WB,IHC	Thorac Cancer; Upregulation of ADAMDEC1 correlates with tumor progression and predicts poor prognosis in non-small cell lung cancer (NSCLC) via the PI3K/AKT pathway.; Authors - Weiliang Zhu