ADRA1A-Specific Polyclonal antibody

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• KD/KO Validated

ADRA1A-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 19777-1-AP

Synonyms

ADRA1A, Alpha-1C adrenergic receptor, Alpha-1A adrenoreceptor, Alpha-1A adrenoceptor, Alpha-1A adrenergic receptor

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Validation Data Gallery

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  WB analysis using 19777-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours without or with blocking peptide (1.4 ug peptide was used to block the binding of 1.0 ug antibody).

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  WB analysis of HeLa using 19777-1-AP

  WB result of ADRA1A-Specific antibody (19777-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADRA1A-Specific transfected HeLa cells.

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  WB analysis using 19777-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 19777-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRA1A-Specific Polyclonal antibody (19777-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, mouse brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

19777-1-AP targets ADRA1A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: adrenergic, alpha-1A-, receptor
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_000680
• Gene Symbol: ADRA1A
• Gene ID (NCBI): 148
• RRID: AB_10643238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRA1A, also named as ADRA1C, belongs to the G-protein coupled receptor 1 family. ADRA1A mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. The antibody is specific to ADRA1A.

Publications

Species	Application	Title
human		Eur J Immunol; Chronic stimulation desensitizes β2-adrenergic receptor responses in natural killer cells; Authors - Martin Jürgens
rat	WB	iScience; Changes in transcriptomic landscape in human end-stage heart failure with distinct etiology.; Authors - Miaomiao Zhu
human	IHC,IF	Pharmaceuticals (Basel); Vascular α1A Adrenergic Receptors as a Potential Therapeutic Target for IPAD in Alzheimer's Disease.; Authors - Miles Frost
human	WB	Vascul Pharmacol; The ERK1/2 pathway participates in the upregulation of the expression of mesenteric artery α1 receptors by intravenous tail injections of mmLDL in mice.; Authors - Jun-Jie Wang
mouse	WB	Heliyon; Luteolin improves vasoconstriction function and survival of septic mice via AMPK/NF-κB pathway; Authors - Bin Liu
rat	WB	BMC Neurosci; Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α2A-adrenergic receptor; Authors - Chaoyong Tian