ADRA1A-Specific Polyclonal antibody

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ADRA1A-Specific Polyclonal Antibody for WB, ELISA

Cat No. 19777-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

ADRA1A, ADRA1C, Alpha-1A adrenergic receptor, Alpha-1A adrenoceptor, Alpha-1A adrenoreceptor

• 

  WB analysis using 19777-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours without or with blocking peptide (1.4 ug peptide was used to block the binding of 1.0 ug antibody).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 19777-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 19777-1-AP

  WB result of ADRA1A-Specific antibody (19777-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADRA1A-Specific transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 19777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 19777-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRA1A-Specific Polyclonal antibody (19777-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, mouse brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19777-1-AP targets ADRA1A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: adrenergic, alpha-1A-, receptor
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_000680
• Gene Symbol: ADRA1A
• Gene ID (NCBI): 148
• RRID: AB_10643238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35348
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRA1A, also named as ADRA1C, belongs to the G-protein coupled receptor 1 family. ADRA1A mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. The antibody is specific to ADRA1A.

Publications

What published studies show

The ADRA1 antibody (Cat# 19777-1-AP) has 12 citations published in journals including Phytotherapy Research, Journal of Ethnopharmacology, Communications Biology, Pharmaceuticals, Biomolecules, iScience, European Journal of Immunology, Heliyon, Vascular Pharmacology, BMC Neuroscience, Biochemical and Biophysical Research Communications, and Neurobiology of Disease. Research fields span Alzheimer's disease and neuroinflammation, cardiovascular biology, uveal melanoma, heart failure, immunology, sepsis, vascular pharmacology, hearing loss, blood pressure regulation, and pain signaling.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Phytother Res; Gastrodin Ameliorates Tau Pathology and BBB Dysfunction in 3xTg-AD Transgenic Mice by Regulating the ADRA1/NF-κB/NLRP3 Pathway to Reduce Neuroinflammation; Authors - Bo Li
mouse	WB	J Ethnopharmacol; Jiajian Shuyu pills effectively ameliorate cognitive impairment via regulating the inflammation of microglia in an Alzheimer's disease mouse model; Authors - Yan Chen
mouse	WB	Commun Biol; A paraventricular hypothalamic control of social stress susceptibility by targeting brown adipose tissue β3 adrenoceptor.; Authors - Xinyu Du
human	IHC,IF	Pharmaceuticals (Basel); Vascular α1A Adrenergic Receptors as a Potential Therapeutic Target for IPAD in Alzheimer's Disease.; Authors - Miles Frost
human	IF	Biomolecules; The Discovery of α-Adrenoceptor Antagonists as a Potential New Treatment Option for Uveal Melanoma.; Authors - Yilan He
rat	WB	iScience; Changes in transcriptomic landscape in human end-stage heart failure with distinct etiology.; Authors - Miaomiao Zhu