ADRA1B antibody (22419-1-PBS)

Filter: AllWBFC (Intra)

WB analysis using 22419-1-AP (same clone as 22419-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22419-1-AP (ADRA1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22419-1-PBS in a different storage buffer formulation.
FC experiment of PC-3 using 22419-1-AP (same clone as 22419-1-PBS)
1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22419-1-PBS in a different storage buffer formulation.
×
1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22419-1-PBS in a different storage buffer formulation.

This product is currently not available for sale.

22419-1-PBS targets ADRA1B in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ADRA1B fusion protein Ag18138 Predict reactive species
Full Name	adrenergic, alpha-1B-, receptor
Calculated Molecular Weight	520 aa, 57 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC136569
Gene Symbol	ADRA1B
Gene ID (NCBI)	147
RRID	AB_2879103
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	P35368
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.