ADRA1B Polyclonal antibody

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  WB analysis using 22419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22419-1-AP (ADRA1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of PC-3 using 22419-1-AP

  1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ADRA1B Polyclonal Antibody for WB, ELISA

Cat No. 22419-1-AP

Publications(4)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Alpha-1B adrenoceptor, Alpha-1B adrenergic receptor, Alpha 1B adrenoreceptor, Alpha 1B adrenoceptor, Alpha 1B adrenergic receptor

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, mouse testis tissue, rat brain tissue
• Positive FC (Intra) detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

22419-1-AP targets ADRA1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADRA1B fusion protein Ag18138
• Full Name: adrenergic, alpha-1B-, receptor
• Calculated Molecular Weight: 520 aa, 57 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC136569
• Gene Symbol: ADRA1B
• Gene ID (NCBI): 147
• RRID: AB_2879103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P35368
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	IHC,WB	Chin Med; Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.; Authors - Xian Zhang
rat	WB	Eur J Pharmacol; Alpha1B-adreneroceptor is involved in norepinephrine-induced pulmonary artery smooth muscle cell proliferation via p38 signaling; Authors - Xiaolin Xiao
human	WB	Mol Med Rep; Stigmasterol exerts antiglioma effects by regulating lipid metabolism; Authors - Ting Wei
human	IHC	Onco Targets Ther; Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.; Authors - Chao Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (07-31-2023)

Good signal in Flow cytometry for endogenous expressing levels and confirmed in overexpressing cell line

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0.4ug/ml

FH

Mohamad (Verified Customer) (07-03-2023)

different bands at different MW

• Applications: Western Blot
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Lung