ADRA1B Polyclonal antibody

ADRA1B Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 22419-1-AP

Synonyms

ADRA1, ADRA1B, Alpha 1B adrenergic receptor, Alpha 1B adrenoceptor, Alpha 1B adrenoreceptor, ALPHA1BAR



Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse testis tissue, rat brain tissue
Positive FC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

22419-1-AP targets ADRA1B in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRA1B fusion protein Ag18138
Full Name adrenergic, alpha-1B-, receptor
Calculated molecular weight 520 aa, 57 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC136569
Gene symbol ADRA1B
Gene ID (NCBI) 147
RRIDAB_2879103
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADRA1B antibody 22419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC,WB

Chin Med

Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.

Authors - Xian Zhang
ratWB

Eur J Pharmacol

Alpha1B-adreneroceptor is involved in norepinephrine-induced pulmonary artery smooth muscle cell proliferation via p38 signaling

Authors - Xiaolin Xiao
humanIHC

Onco Targets Ther

Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.

Authors - Chao Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Andrea (Verified Customer) (07-31-2023)

Good signal in Flow cytometry for endogenous expressing levels and confirmed in overexpressing cell line

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 0.4ug/ml
FH

Mohamad (Verified Customer) (07-03-2023)

different bands at different MW

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Lung