AFG3L2 Polyclonal antibody, PBS Only

• 

  WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

  WB result of AFG3L2 antibody (14631-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFG3L2 transfected HeLa cells. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 14631-1-AP (same clone as 14631-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 14631-1-AP (same clone as 14631-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

  IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14631-1-AP (same clone as 14631-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

AFG3L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14631-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

---

Product Information

14631-1-PBS targets AFG3L2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6209

  Product name: Recombinant human AFG3L2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 449-797 aa of BC065016

  Sequence: ILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN

• Full Name: AFG3 ATPase family gene 3-like 2 (yeast)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC065016
• Gene Symbol: AFG3L2
• Gene ID (NCBI): 10939
• RRID: AB_2242420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y4W6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).