AFG3L2 Polyclonal antibody

• Featured Product
• KD/KO Validated

AFG3L2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (1)

Cat no : 14631-1-AP

Synonyms

AFG3 like protein 2, AFG3L2, Paraplegin like protein

---

Validation Data Gallery

• 

  WB analysis using 14631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14631-1-AP

  WB result of AFG3L2 antibody (14631-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFG3L2 transfected HeLa cells.

• 

  WB analysis using 14631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14631-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14631-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14631-1-AP

  IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:5000) with HeLa cells lysate 1560 ug.

• 

  IHC staining of human kidney using 14631-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 14631-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 14631-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14631-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14631-1-AP

  Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 14631-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, mouse kidney tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 25 publications below
• IP: See 2 publications below

Product Information

14631-1-AP targets AFG3L2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AFG3L2 fusion protein Ag6209
• Full Name: AFG3 ATPase family gene 3-like 2 (yeast)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC065016
• Gene Symbol: AFG3L2
• Gene ID (NCBI): 10939
• RRID: AB_2242420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

Publications

Species	Application	Title
mouse	WB	Mol Cell; Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex; Authors - Chen-Wei Tsai ; KD Validated
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
mouse	WB	Research (Wash D C); LonP1 Links Mitochondria-ER Interaction to Regulate Heart Function; Authors - Yujie Li
human	WB	Brain; Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.; Authors - Gerald Pfeffer
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

• Applications: Western Blot