AGR2 Polyclonal antibody

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  WB analysis using 12275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 12275-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12275-1-AP

  IP result of anti-AGR2 (IP:12275-1-AP, 4ug; Detection:12275-1-AP 1:1000) with A549 cells lysate 1200 ug.

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  IHC staining of human breast cancer using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12275-1-AP (AGR2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12275-1-AP (AGR2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

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  IHC staining of human colon using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

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  IF Staining of A549 using 12275-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AGR2 antibody (12275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 12275-1-AP

  1x10^6 A549 cells were intracellularly stained with 0.4 ug AGR2 Polyclonal antibody (12275-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

AGR2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12275-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

GOB4, GOB 4, AGR 2, AG-2, AG2

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Tested Applications

• Positive WB detected in: A549 cells, rat colon tissue, rat stomach tissue, mouse stomach tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 9 publications below
• IF: See 5 publications below

Product Information

12275-1-AP targets AGR2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGR2 fusion protein Ag2919
• Full Name: anterior gradient homolog 2 (Xenopus laevis)
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC015503
• Gene Symbol: AGR2
• Gene ID (NCBI): 10551
• RRID: AB_2225096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95994
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia.; Authors - Cindrilla Chumduri
human,mouse	IHC	Nat Protoc; Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.; Authors - Rajendra Kumar Gurumurthy
human	IHC	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	WB,IHC	Proc Natl Acad Sci U S A; A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer.; Authors - Herfs Michael M
human	IHC	J Pathol; A novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia.; Authors - Michael Herfs
human	IHC	Int J Cancer; Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.; Authors - Michael Herfs