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AGR2 Polyclonal antibody

AGR2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12275-1-AP


AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2

Tested Applications

Positive WB detected inA549 cells, rat colon tissue, rat stomach tissue, mouse stomach tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12275-1-AP targets AGR2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGR2 fusion protein Ag2919
Full Name anterior gradient homolog 2 (Xenopus laevis)
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight 17-20 kDa
GenBank accession numberBC015503
Gene symbol AGR2
Gene ID (NCBI) 10551
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.


Product Specific Protocols
WB protocol for AGR2 antibody 12275-1-APDownload protocol
IHC protocol for AGR2 antibody 12275-1-APDownload protocol
IP protocol for AGR2 antibody 12275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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