• Featured Product
  • KD/KO Validated

AGR2 Antibody

AGR2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12275-1-AP

Synonyms

AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2



Tested Applications

Positive WB detected inmouse stomach tissue, human colon tissue, human stomach tissue, mouse small intestine tissue, rat colon tissue, rat stomach tissue, SW 1990 cells
Positive IP detected inSW 1990 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12275-1-AP targets AGR2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGR2 fusion protein Ag2919
Full Name anterior gradient homolog 2 (Xenopus laevis)
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight 17-20 kDa
GenBank accession numberBC015503
Gene symbol AGR2
Gene ID (NCBI) 10551
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.

Protocols

Product Specific Protocols
WB protocol for AGR2 antibody 12275-1-APDownload protocol
IHC protocol for AGR2 antibody 12275-1-APDownload protocol
IP protocol for AGR2 antibody 12275-1-APDownload protocol
FC protocol for AGR2 antibody 12275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Lett

Anterior Gradient-2 monoclonal antibody inhibits lung cancer growth and metastasis by upregulating p53 pathway and without exerting any toxicological effects: A preclinical study.

Authors - Hema Negi
humanWB

EMBO Mol Med

Control of anterior GRadient 2 (AGR2) dimerization links endoplasmic reticulum proteostasis to inflammation.

Authors - Marion Maurel
  • KD Validated
mouseIF

J Diabetes Investig

Early administration of dapagliflozin preserves pancreatic beta-cell mass through a legacy effect in type 2 diabetic mice.

Authors - Ayumi Kanno
humanIHC

Tumour Biol

Identification and prognostic value of anterior gradient protein 2 expression in breast cancer based on tissue microarray.

Authors - Jilong Guo
humanIHC

Am J Surg Pathol

Cervical squamocolumnar junction-specific markers define distinct, clinically relevant subsets of low-grade squamous intraepithelial lesions.

Authors - Michael Herfs
humanWB

J Proteome Res

Identification of serum biomarkers for colorectal cancer metastasis using a differential secretome approach.

Authors - Xue Hua H