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AGR2 Polyclonal antibody
AGR2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, ELISA
Cat no : 12275-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, rat colon tissue, rat stomach tissue, mouse stomach tissue|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human breast cancer tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 3 publications below|
|IHC||See 8 publications below|
|IF||See 2 publications below|
12275-1-AP targets AGR2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AGR2 fusion protein Ag2919|
|Full Name||anterior gradient homolog 2 (Xenopus laevis)|
|Calculated molecular weight||175 aa, 20 kDa|
|Observed molecular weight||17-20 kDa|
|GenBank accession number||BC015503|
|Gene ID (NCBI)||10551|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.
|Product Specific Protocols|
|WB protocol for AGR2 antibody 12275-1-AP||Download protocol|
|IHC protocol for AGR2 antibody 12275-1-AP||Download protocol|
|IP protocol for AGR2 antibody 12275-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nat Cell Biol
Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia.
Proc Natl Acad Sci U S A
A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer.
A novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia.
Int J Cancer
Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.
J Proteome Res
Identification of serum biomarkers for colorectal cancer metastasis using a differential secretome approach.
Am J Surg Pathol
Cervical squamocolumnar junction-specific markers define distinct, clinically relevant subsets of low-grade squamous intraepithelial lesions.