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AKAP13 Polyclonal antibody, PBS Only (27947-1-PBS)

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WB analysis using 27947-1-AP (same clone as 27947-1-PBS)
HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 27947-1-AP (AKAP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 27947-1-AP (same clone as 27947-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 27947-1-AP (same clone as 27947-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 27947-1-AP (same clone as 27947-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 27947-1-AP (same clone as 27947-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

AKAP13 Polyclonal antibody, PBS Only

Name: AKAP13 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 27947-1-PBS

AKAP13 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27947-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

A kinase anchor protein 13, AKAP 13, A-kinase anchor protein 13, ARHGEF13, BRX

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

27947-1-PBS targets AKAP13 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27486 Product name: Recombinant human AKAP13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 359-587 aa of NM_001270546 Sequence: MIVEEENTDRSCRKKNKGVERKGEEVEPAPIVDSGTVSDQDSCLQSLPDCGVKGTEGLSSCGNRNEETGTKSSGMPTDQESLSSGDAVLQRDLVMEPGTAQYSSGGELGGISTTNVSTPDTAGEMEHGLMNPDATVWKNVLQGGESTKERFENSNIGTAGASDVHVTSKPVDKISVPNCAPAASSLDGNKPAESSLAFSNEETSTEKTAETETSRSREESADAPVDQNSV Predict reactive species
Full Name	A kinase (PRKA) anchor protein 13
Calculated Molecular Weight	308 kDa
Observed Molecular Weight	308 kDa
GenBank Accession Number	NM_001270546
Gene Symbol	AKAP13
Gene ID (NCBI)	11214
RRID	AB_2881017
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12802
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions