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  • KD/KO Validated

ALY Polyclonal antibody

ALY Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16690-1-AP

Synonyms

Ally of AML 1 and LEF 1, ALY, ALYREF, BEF, bZIP enhancing factor BEF, THO complex 4, THO complex subunit 4, Tho4, THOC4



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A431 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16690-1-AP targets ALY in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALY fusion protein Ag10044
Full Name THO complex 4
Calculated Molecular Weight 257 aa, 27 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC052302
Gene Symbol THOC4
Gene ID (NCBI) 10189
RRIDAB_2878300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ALY antibody 16690-1-APDownload protocol
IHC protocol for ALY antibody 16690-1-APDownload protocol
IF protocol for ALY antibody 16690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
pigWB

ACS Infect Dis

Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.

Authors - Lin Yang
  • KD Validated
humanWB,IF

PLoS Pathog

TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus

Authors - Lingcai Zhao
  • KD Validated
humanWB

Nature

mRNA recognition and packaging by the human transcription-export complex

Authors - Belén Pacheco-Fiallos
humanWB

Cell Death Dis

Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses

Authors - Shuang Ding
humanRIP

Phytomedicine

Niujiao Dihuang Jiedu decoction promotes SLC7A11 m5C methylation modification against ferroptosis in acute-on-chronic liver failure

Authors - Liqiao Huang