ALY Polyclonal antibody

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  WB analysis of HeLa using 16690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16690-1-AP

  WB result of ALY antibody (16690-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALY transfected HeLa cells.

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  WB analysis of HEK-293 using 16690-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 16690-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16690-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16690-1-AP (ALY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16690-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16690-1-AP (ALY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ALY antibody (16690-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

ALY Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16690-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, RIP, ELISA

Synonyms

Ally of AML 1 and LEF 1, ALY, ALYREF, BEF, bZIP enhancing factor BEF, THO complex 4, THO complex subunit 4, Tho4, THOC4

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, A431 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

16690-1-AP targets ALY in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10044

  Product name: Recombinant human ALY protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 5-257 aa of BC052302

  Sequence: MDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIARGAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRNRGAGGFGGGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS

• Full Name: THO complex 4
• Calculated Molecular Weight: 257 aa, 27 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC052302
• Gene Symbol: ALY
• Gene ID (NCBI): 10189
• RRID: AB_2878300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86V81
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
pig	WB	ACS Infect Dis; Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.; Authors - Lin Yang ; KD Validated
human	WB,IF	PLoS Pathog; TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus; Authors - Lingcai Zhao ; KD Validated
human	WB	Nature; mRNA recognition and packaging by the human transcription-export complex; Authors - Belén Pacheco-Fiallos
human	WB	Cell Death Dis; Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses; Authors - Shuang Ding
human	RIP	Phytomedicine; Niujiao Dihuang Jiedu decoction promotes SLC7A11 m5C methylation modification against ferroptosis in acute-on-chronic liver failure; Authors - Liqiao Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Weak band on WB from HeLa cells extracts but specific for ALYREF/THOC4 (1/1000 dilution, Overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa