AMMECR1 Polyclonal antibody

AMMECR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24687-1-AP

Synonyms

AMMECR1, AMMERC1

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Validation Data Gallery

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  WB analysis of Jurkat using 24687-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24687-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 24687-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 24687-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24687-1-AP (AMMECR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 24687-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24687-1-AP (AMMECR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse liver tissue, mouse uterus tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24687-1-AP is AMMECR1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMMECR1 fusion protein Ag16977
• Full Name: Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1
• Calculated Molecular Weight: 330 aa, 35 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC060813
• Gene Symbol: AMMECR1
• Gene ID (NCBI): 9949
• RRID: AB_2879671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMMECR1 also named as AMME syndrome candidate gene 1 protein is a 333 amino acid protein, which contains 1 AMMECER1 domain. Containing a glycine-rich N terminus, the AMMECR1 protein exhibits putative nuclear localization and a substantial level of instability, suggesting it plays a role in regulation. The deletion of AMMECR1 gene may be involved in glomerulonephritis, sensorineural hearing loss, mental retardation, midface hypoplasia and elliptocytosis.