APC1 Polyclonal antibody

APC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21748-1-AP

Synonyms

ANAPC1, APC1, Mitotic checkpoint regulator, TSG24



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HT-1080 cells, K-562 cells, mouse brain tissue, Transfected HEK-293 cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 21748-1-AP is APC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APC1 fusion protein Ag13399
Full Name anaphase promoting complex subunit 1
Calculated molecular weight 1944 aa, 217 kDa
Observed molecular weight 200-210 kDa
GenBank accession numberBC104902
Gene symbol ANAPC1
Gene ID (NCBI) 64682
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APC1 antibody 21748-1-APDownload protocol
IHC protocol for APC1 antibody 21748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

Authors - Jianguo Yang
humanWB

Sci Rep

Reciprocal interaction between SIRT6 and APC/C regulates genomic stability.

Authors - Helin Wang
humanWB

Oncotarget

Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.

Authors - Jin Zhu