APC1 Polyclonal antibody

APC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21748-1-AP

Synonyms

ANAPC1, APC1, Mitotic checkpoint regulator, TSG24



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HT-1080 cells, K-562 cells, mouse brain tissue, Transfected HEK-293 cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

The immunogen of 21748-1-AP is APC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APC1 fusion protein Ag13399
Full Name anaphase promoting complex subunit 1
Calculated Molecular Weight 1944 aa, 217 kDa
Observed Molecular Weight 200-210 kDa
GenBank Accession NumberBC104902
Gene Symbol ANAPC1
Gene ID (NCBI) 64682
RRIDAB_10733241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APC1 antibody 21748-1-APDownload protocol
IHC protocol for APC1 antibody 21748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
humanWB

Oncotarget

Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.

Authors - Jin Zhu
humanWB

Sci Rep

Reciprocal interaction between SIRT6 and APC/C regulates genomic stability.

Authors - Helin Wang
humanWB

Cell Rep

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

Authors - Jianguo Yang