CoraLite® Plus 750-conjugated ANO4 Recombinant antibody

• 

  WB analysis using CL750-82970

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82970 (ANO4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ANO4 Uni-rAb^TM Recombinant Antibody for WB

Cat No. CL750-82970

Clone No.230308G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Synonyms

Transmembrane protein 16D, TMEM16D, Anoctamin-4, anoctamin 4, 230308G1

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82970 targets ANO4 in WB applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: anoctamin 4
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 100-111 kDa
• GenBank Accession Number: NM_178826
• Gene Symbol: ANO4
• Gene ID (NCBI): 121601
• RRID: AB_3673756
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q32M45
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ANO4, also named as TMEM16D, belongs to the anoctamin family. ANO4 is primarily expressed in the central nervous system and certain endocrine glands, and it has been linked to a variety of neurological disorders (PMID: 36836529). The antibody recognize the isoform1 and isoform2 of ANO4.