ANO4 Recombinant antibody

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  WB analysis using 82970-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82970-1-RR (ANO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82970-1-RR

  WB result of ANO4 antibody (82970-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANO4 transfected HeLa cells.

• Featured Product
• KD/KO Validated

ANO4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 82970-1-RR

Clone No.230308G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Transmembrane protein 16D, TMEM16D, Anoctamin-4, anoctamin 4, 230308G1

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, PC-3 cells, SH-SY5Y cells, mouse testis tissue, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82970-1-RR targets ANO4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: anoctamin 4
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 100-111 kDa
• GenBank Accession Number: NM_178826
• Gene Symbol: ANO4
• Gene ID (NCBI): 121601
• RRID: AB_3670718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q32M45
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANO4, also named as TMEM16D, belongs to the anoctamin family. ANO4 is primarily expressed in the central nervous system and certain endocrine glands, and it has been linked to a variety of neurological disorders (PMID: 36836529). The antibody recognize the isoform1 and isoform2 of ANO4.