• Featured Product
  • KD/KO Validated

AP50 Polyclonal antibody

AP50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27355-1-AP

Synonyms

Adaptin mu2, AP 2 complex subunit mu, AP 2 mu chain, AP2M1, AP50, CLAPM1, HA2 50 kDa subunit, KIAA0109, mu2



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27355-1-AP is AP50 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP50 fusion protein Ag26059
Full Name adaptor-related protein complex 2, mu 1 subunit
Calculated Molecular Weight 433 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC013796
Gene Symbol AP2M1
Gene ID (NCBI) 1173
RRIDAB_2880853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AP50 antibody 27355-1-APDownload protocol
IHC protocol for AP50 antibody 27355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

Autophagy

AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.

Authors - Ashwinkumar Subramenium Ganapathy
  • KO Validated
humanIHC

Oral Dis

Expression levels of SIX1, ME2, and AP2M1 in adenoid cystic carcinoma and mucoepidermoid carcinoma.

Authors - Cong-Cong Wu
humanIHC

Cancer Res

Lipid metabolic reprogramming extends beyond histological tumor demarcations in operable human pancreatic cancer

Authors - Juho Pirhonen
mouseWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu
humanIHC

J Transl Med

A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer

Authors - Huixia Huang