APJ/APLNR Polyclonal antibody

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  WB analysis of SH-SY5Y using 20341-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20341-1-AP (APJ antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 20341-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug APJ Polyclonal antibody (20341-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

APJ/APLNR Polyclonal Antibody for WB, ELISA

Cat No. 20341-1-AP

Publications(44)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

APJ, AGTRL1, Angiotensin receptor-like 1, APLNR, G-protein coupled receptor APJ

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 34 publications below
• IHC: See 8 publications below
• IF: See 15 publications below
• FC: See 1 publications below

Product Information

20341-1-AP targets APJ/APLNR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APJ/APLNR fusion protein Ag14138
• Full Name: apelin receptor
• Calculated Molecular Weight: 380 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC032688
• Gene Symbol: APJ
• Gene ID (NCBI): 187
• RRID: AB_2878676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35414
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APJ, also named APLNR and AGTRL1, belongs to the G-protein coupled receptor 1 family. It is a receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. APJ is an alternative coreceptor with CD4 for HIV-1 infection. It may be involved in the development of AIDS dementia. APJ is expressed abundantly in the corpus callosum and spinal cord.

Publications

Species	Application	Title
human	IF	Immunity; Single-cell atlas of endothelial and mural cells across primary and metastatic brain tumors; Authors - Leire Bejarano
mouse	IHC	Cell Metab; Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis.; Authors - Katerina Rohlenova
mouse	IF	Sci Transl Med; Apelin stimulation of the vascular skeletal muscle stem cell niche enhances endogenous repair in dystrophic mice; Authors - Emmeran Le Moal
mouse	IF	Nat Commun; Molecular determinants of nephron vascular specialization in the kidney.; Authors - David M Barry
mouse	WB	Int J Nanomedicine; Apelin-13-Loaded Macrophage Membrane-Encapsulated Nanoparticles for Targeted Ischemic Stroke Therapy via Inhibiting NLRP3 Inflammasome-Mediated Pyroptosis; Authors - Chang-Sheng Ma
human,mouse	WB	Free Radic Biol Med; The Elabela-APJ axis attenuates sepsis-induced myocardial dysfunction by reducing pyroptosis by balancing the formation and degradation of autophagosomes; Authors - Shuai Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nicole (Verified Customer) (02-24-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK cells