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APJ Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 20341-1-AP

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Con: 61 μg/150 μl

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Tested Applications

Applications:
WB, IHC, FC, ELISA
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Images See all

APJ Antibody WB SH-SY5Y cells 20341-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20341-1-AP( APJ antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

APJ Antibody IHC human colon tissue 20341-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 20341-1-AP( APJ antibody at dilution of 1:50 (under 40x lens)

APJ Antibody IHC human colon tissue 20341-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 20341-1-AP( APJ antibody at dilution of 1:50 (under 10x lens)

APJ Antibody FC SH-SY5Y cells 20341-1-AP

1X10^6 SH-SY5Y cells were stained with 0.2ug APJ antibody (20341-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
SH-SY5Y cells
Positive IHC detected in:
human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
APJ fusion protein Ag14138
Full name:
apelin receptor
Calculated molecular weight:
380aa,43 kDa
Observed molecular weight:
43 kDa
GenBank accession number:
BC032688
Gene ID (NCBI):
187
Gene symbol
APLNR
Synonyms
AGTRL1, Angiotensin receptor like 1, apelin receptor, APJ, APJR, APLNR, G protein coupled receptor APJ

Background

APJ, also named as APLNR and AGTRL1, belongs to the G-protein coupled receptor 1 family. It is a receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. APJ is an alternative coreceptor with CD4 for HIV-1 infection. It may be involved in the development of AIDS dementia. APJ is expressed abundantly in the corpus callosum and spinal cord.


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