APJ Polyclonal antibody

• Featured Product
• KD/KO Validated

APJ Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 20341-1-AP

Synonyms

AGTRL1, Angiotensin receptor like 1, apelin receptor, APJ, APJR, APLNR, G protein coupled receptor APJ

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Validation Data Gallery

• 

  WB analysis of SH-SY5Y using 20341-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20341-1-AP (APJ antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 20341-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug APJ antibody (20341-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Product Information

The immunogen of 20341-1-AP is APJ Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APJ fusion protein Ag14138
• Full Name: apelin receptor
• Calculated molecular weight: 380 aa, 43 kDa
• Observed molecular weight: 43 kDa
• GenBank accession number: BC032688
• Gene symbol: APLNR
• Gene ID (NCBI): 187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APJ, also named as APLNR and AGTRL1, belongs to the G-protein coupled receptor 1 family. It is a receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. APJ is an alternative coreceptor with CD4 for HIV-1 infection. It may be involved in the development of AIDS dementia. APJ is expressed abundantly in the corpus callosum and spinal cord.

Publications

Species	Application	Title
mouse	IHC	Cell Metab; Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis.; Authors - Katerina Rohlenova
mouse	IF	Nat Commun; Molecular determinants of nephron vascular specialization in the kidney.; Authors - David M Barry
rat	WB	Free Radic Biol Med; Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway; Authors - Liang Chen
mouse	WB	EBioMedicine; Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.; Authors - Jun Seok Son
rat	WB	Stem Cell Res Ther; ELABELA ameliorates hypoxic/ischemic-induced bone mesenchymal stem cell apoptosis via alleviation of mitochondrial dysfunction and activation of PI3K/AKT and ERK1/2 pathways.; Authors - Jiaying Fu ; KD Validated
human	WB	FASEB J; APLNR is involved in ATRA-induced growth inhibition of nasopharyngeal carcinoma and may suppress EMT through PI3K-Akt-mTOR signaling.; Authors - Yi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicole (Verified Customer) (02-24-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK cells

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