• Featured Product
  • KD/KO Validated

APJ Polyclonal antibody

APJ Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20341-1-AP

Synonyms

AGTRL1, Angiotensin receptor like 1, apelin receptor, APJ, APJR, APLNR, G protein coupled receptor APJ



Tested Applications

Positive WB detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20341-1-AP is APJ Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APJ fusion protein Ag14138
Full Name apelin receptor
Calculated molecular weight 380 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC032688
Gene symbol APLNR
Gene ID (NCBI) 187
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APJ, also named as APLNR and AGTRL1, belongs to the G-protein coupled receptor 1 family. It is a receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. APJ is an alternative coreceptor with CD4 for HIV-1 infection. It may be involved in the development of AIDS dementia. APJ is expressed abundantly in the corpus callosum and spinal cord.

Protocols

Product Specific Protocols
WB protocol for APJ antibody 20341-1-APDownload protocol
FC protocol for APJ antibody 20341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Metab

Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis.

Authors - Katerina Rohlenova
mouseIF

Nat Commun

Molecular determinants of nephron vascular specialization in the kidney.

Authors - David M Barry
ratWB

Free Radic Biol Med

Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway

Authors - Liang Chen
mouseWB

EBioMedicine

Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.

Authors - Jun Seok Son
ratWB

Stem Cell Res Ther

ELABELA ameliorates hypoxic/ischemic-induced bone mesenchymal stem cell apoptosis via alleviation of mitochondrial dysfunction and activation of PI3K/AKT and ERK1/2 pathways.

Authors - Jiaying Fu
  • KD Validated
humanWB

FASEB J

APLNR is involved in ATRA-induced growth inhibition of nasopharyngeal carcinoma and may suppress EMT through PI3K-Akt-mTOR signaling.

Authors - Yi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nicole (Verified Customer) (02-24-2022)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK cells