APJ Polyclonal antibody

APJ Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20341-1-AP

Synonyms

AGTRL1, Angiotensin receptor like 1, apelin receptor, APJ, APJR, APLNR, G protein coupled receptor APJ



Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20341-1-AP is APJ Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APJ fusion protein Ag14138
Full Name apelin receptor
Calculated molecular weight 380 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC032688
Gene symbol APLNR
Gene ID (NCBI) 187
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APJ, also named as APLNR and AGTRL1, belongs to the G-protein coupled receptor 1 family. It is a receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. APJ is an alternative coreceptor with CD4 for HIV-1 infection. It may be involved in the development of AIDS dementia. APJ is expressed abundantly in the corpus callosum and spinal cord.

Protocols

Product Specific Protocols
WB protocol for APJ antibody 20341-1-APDownload protocol
IHC protocol for APJ antibody 20341-1-APDownload protocol
FC protocol for APJ antibody 20341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuroscience

The Hippocampus is a Critical Site Mediating Antidepressant-like Activity of Apelin-13 in Rats.

Authors - Zhi-Yong Xiao
ratWB

Neuroscience

Apelin-13 Upregulates BDNF Against Chronic Stress-induced Depression-like Phenotypes by Ameliorating HPA Axis and Hippocampal Glucocorticoid Receptor Dysfunctions.

Authors - Ting-Ting Dai
ratWB

Front Pharmacol

Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer's Disease Through Activation of BDNF-TrkB Signaling Pathway.

Authors - Huaiqing Luo
humanWB,IHC

Amino Acids

Apelin attenuates the osteoblastic differentiation of aortic valve interstitial cells via the ERK and PI3-K/Akt pathways.

Authors - Zhao-Shun Yuan
humanWB

J Dermatol Sci

Rip 1-dependent endothelial necroptosis participates in ischemia-reperfusion injury of mouse flap.

Authors - Lele Geng
mouseIF

Nat Commun

Molecular determinants of nephron vascular specialization in the kidney.

Authors - David M Barry