APOE Monoclonal antibody

APOE Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse







Cat no : 66830-1-Ig


AD2, Apo E, APOE, apolipoprotein E, LPG

Tested Applications

Positive WB detected inhuman plasma tissue, human blood, human plasma, T-47D cells, Caco-2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:3000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66830-1-Ig targets APOE in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOE fusion protein Ag28186
Full Name apolipoprotein E
Calculated molecular weight 36 kDa
Observed molecular weight 34-36 kDa
GenBank accession numberBC003557
Gene symbol APOE
Gene ID (NCBI) 348
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.


Product Specific Protocols
WB protocol for APOE antibody 66830-1-IgDownload protocol
IHC protocol for APOE antibody 66830-1-IgDownload protocol
IF protocol for APOE antibody 66830-1-IgDownload protocol
FC protocol for APOE antibody 66830-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




A critical role of VMP1 in lipoprotein secretion.

Authors - Hideaki Morishita

J Biol Chem

Lack of ApoE inhibits ADan amyloidosis in a mouse model of Familial Danish Dementia

Authors - Anllely Fernandez

Front Immunol

Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.

Authors - Anika Alberts

Mol Oncol

Single-cell and spatial analyses reveal the association between gene expression of glutamine synthetase with the immunosuppressive phenotype of APOE+CTSZ+TAM in cancers

Authors - Jinfen Wei

Nat Neurosci

Lipid-accumulated reactive astrocytes promote disease progression in epilepsy

Authors - Zhang-Peng Chen

Cancer Sci

Discovery of lipid profiles in plasma-derived extracellular vesicles as biomarkers for breast cancer diagnosis

Authors - Lin Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Silvia (Verified Customer) (12-03-2019)

Specific antibody for IF and WB on Human tissues

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: WB 1:3000, IF 1:500
  • Cell Tissue Type: Human organoids