|Positive WB detected in
|human plasma tissue, human blood, human plasma, T-47D cells, Caco-2 cells
|Positive IHC detected in
|mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:3000-1:20000
|IHC : 1:50-1:500
|IF : 1:400-1:1600
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66830-1-Ig targets APOE in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Mouse / IgG1
|APOE fusion protein Ag28186
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.
|Product Specific Protocols
|WB protocol for APOE antibody 66830-1-Ig
|IHC protocol for APOE antibody 66830-1-Ig
|IF protocol for APOE antibody 66830-1-Ig
|FC protocol for APOE antibody 66830-1-Ig
|Click here to view our Standard Protocols
A critical role of VMP1 in lipoprotein secretion.
J Biol Chem
Lack of ApoE inhibits ADan amyloidosis in a mouse model of Familial Danish Dementia
Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.
Single-cell and spatial analyses reveal the association between gene expression of glutamine synthetase with the immunosuppressive phenotype of APOE+CTSZ+TAM in cancers
Lipid-accumulated reactive astrocytes promote disease progression in epilepsy
Discovery of lipid profiles in plasma-derived extracellular vesicles as biomarkers for breast cancer diagnosis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Silvia (Verified Customer) (12-03-2019)
Specific antibody for IF and WB on Human tissues