APOE Monoclonal antibody

APOE Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B2C9

Cat no : 66830-1-Ig

Synonyms

AD2, Apo E, APOE, apolipoprotein E, LPG



Tested Applications

Positive WB detected inhuman plasma tissue, human blood tissue, T-47D cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66830-1-Ig targets APOE in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOE fusion protein Ag28186
Full Name apolipoprotein E
Calculated molecular weight 36 kDa
Observed molecular weight 34-36 kDa
GenBank accession numberBC003557
Gene symbol APOE
Gene ID (NCBI) 348
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

Protocols

Product Specific Protocols
WB protocol for APOE antibody 66830-1-IgDownload protocol
IHC protocol for APOE antibody 66830-1-IgDownload protocol
IF protocol for APOE antibody 66830-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.

Authors - Anika Alberts
humanWB,IF

Elife

A critical role of VMP1 in lipoprotein secretion.

Authors - Hideaki Morishita

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Silvia (Verified Customer) (12-03-2019)

Specific antibody for IF and WB on Human tissues

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: WB 1:3000, IF 1:500
  • Cell Tissue Type: Human organoids