APOE Monoclonal antibody, PBS Only

APOE Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2C9

Cat no : 66830-1-PBS

Synonyms

AD2, Apo E, APOE, apolipoprotein E, LPG

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Validation Data Gallery

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  WB analysis of human plasma using 66830-1-Ig (same clone as 66830-1-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at a range of dilutions from 1:5000 to 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

  WB result of APOE antibody (66830-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 66830-1-Ig (same clone as 66830-1-PBS)

  human blood was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66830-1-Ig (APOE antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APOE (66830-1-Ig, Clone:1B2C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66830-1-PBS targets APOE in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: APOE fusion protein Ag28186
• Full Name: apolipoprotein E
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-36 kDa
• GenBank Accession Number: BC003557
• Gene Symbol: APOE
• Gene ID (NCBI): 348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.