Apolipoprotein H Polyclonal antibody
Apolipoprotein H Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11892-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human blood tissue, mouse testis tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 1 publications below |
IP | See 1 publications below |
Product Information
11892-1-AP targets Apolipoprotein H in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Apolipoprotein H fusion protein Ag2500 |
Full Name | apolipoprotein H (beta-2-glycoprotein I) |
Calculated molecular weight | 345 aa, 38 kDa |
Observed molecular weight | 43-55 kDa |
GenBank accession number | BC026283 |
Gene symbol | APOH |
Gene ID (NCBI) | 350 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.
Protocols
Product Specific Protocols | |
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WB protocol for Apolipoprotein H antibody 11892-1-AP | Download protocol |
IHC protocol for Apolipoprotein H antibody 11892-1-AP | Download protocol |
IF protocol for Apolipoprotein H antibody 11892-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun ATRX, DAXX or MEN1 mutant pancreatic neuroendocrine tumors are a distinct alpha-cell signature subgroup. | ||
Neuropharmacology Anti-β2GPI/β2GPI induces neutrophil extracellular traps formation to promote thrombogenesis via the TLR4/MyD88/MAPKs axis activation. |