Apolipoprotein H Polyclonal antibody

Apolipoprotein H Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11892-1-AP

Synonyms

Anticardiolipin cofactor, APC inhibitor, Apo H, APOH, Apolipoprotein H, B2G1, B2GPI, Beta 2 glycoprotein 1, Beta 2 glycoprotein I, BG



Tested Applications

Positive WB detected inhuman blood tissue, mouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11892-1-AP targets Apolipoprotein H in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein H fusion protein Ag2500
Full Name apolipoprotein H (beta-2-glycoprotein I)
Calculated molecular weight 345 aa, 38 kDa
Observed molecular weight 43-55 kDa
GenBank accession numberBC026283
Gene symbol APOH
Gene ID (NCBI) 350
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein H antibody 11892-1-APDownload protocol
IHC protocol for Apolipoprotein H antibody 11892-1-APDownload protocol
IF protocol for Apolipoprotein H antibody 11892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Neuropharmacology

Anti-β2GPI/β2GPI induces neutrophil extracellular traps formation to promote thrombogenesis via the TLR4/MyD88/MAPKs axis activation.

Authors - Caijun Zha
humanIHC

Nat Commun

ATRX, DAXX or MEN1 mutant pancreatic neuroendocrine tumors are a distinct alpha-cell signature subgroup.

Authors - Chang S Chan