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- KD/KO Validated
Aquaporin 4 Polyclonal antibody
Aquaporin 4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, IF, FC, ELISA
Cat no : 16473-1-AP
Validation Data Gallery
|Positive WB detected in||mouse heart tissue, SKOV-3 cells, mouse kidney tissue, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||mouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||rat brain tissue, mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:2500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 35 publications below|
|IHC||See 15 publications below|
|IF||See 18 publications below|
|IP||See 1 publications below|
|FC||See 1 publications below|
16473-1-AP targets Aquaporin 4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Aquaporin 4 fusion protein Ag9561|
|Full Name||aquaporin 4|
|Calculated molecular weight||323 aa, 35 kDa|
|Observed molecular weight||35-37 kDa, 32-34 kDa|
|GenBank accession number||BC022286|
|Gene ID (NCBI)||361|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
|Product Specific Protocols|
|WB protocol for Aquaporin 4 antibody 16473-1-AP||Download protocol|
|IHC protocol for Aquaporin 4 antibody 16473-1-AP||Download protocol|
|IF protocol for Aquaporin 4 antibody 16473-1-AP||Download protocol|
|IP protocol for Aquaporin 4 antibody 16473-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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