Tested Applications
| Positive WB detected in | mouse liver tissue, SKOV-3 cells, mouse kidney tissue, mouse heart tissue, mouse brain tissue, rat brain tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:2500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 62 publications below |
| IHC | See 21 publications below |
| IF | See 41 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
Product Information
16473-1-AP targets Aquaporin 4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Aquaporin 4 fusion protein Ag9561 Predict reactive species |
| Full Name | Aquaporin 4 |
| Calculated Molecular Weight | 323 aa, 35 kDa |
| Observed Molecular Weight | 35-37 kDa, 32-34 kDa |
| GenBank Accession Number | BC022286 |
| Gene Symbol | Aquaporin 4 |
| Gene ID (NCBI) | 361 |
| RRID | AB_2827426 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55087 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
| IHC protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
| IF protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
| IP protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Cancer Res A Histopathologic Correlation Study Evaluating Glymphatic Function in Brain Tumors by Multi-Parametric MRI | ||
Brain Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia. | ||
Brain Behav Immun Polyunsaturated fatty acid supplement alleviates depression-incident cognitive dysfunction by protecting the cerebrovascular and glymphatic systems. | ||
Glia Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice. | ||
Brain Behav Immun Hypoxia augments LPS-induced inflammation and triggers high altitude cerebral edema in mice. | ||
J Neuroinflammation Regulatory T cells protect against brain damage by alleviating inflammatory response in neuromyelitis optica spectrum disorder. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Paula (Verified Customer) (07-30-2024) | Worked well for human primary cell line, dilution used 1:500 (secondary 1:400)
 |
FH aidan (Verified Customer) (07-27-2024) | Very happy with this antibody would highly recommend. Overnight 4degrees, 1:2000. Bis tris gel. Secondary 1:4000 HRP 1h RT.
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FH Reyes (Verified Customer) (04-05-2024) | Aquaporin 4 (in green) shows a strong stainning, although difficult for the analyses, in human FFPE brain cortex.
 |
