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- KD/KO Validated
Aquaporin 4 Polyclonal antibody
Aquaporin 4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 16473-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, SKOV-3 cells, mouse kidney tissue, mouse brain tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:2500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 29 publications below |
| IHC | See 14 publications below |
| IF | See 16 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 16473-1-AP is Aquaporin 4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Aquaporin 4 fusion protein Ag9561 |
| Full Name | aquaporin 4 |
| Calculated molecular weight | 323 aa, 35 kDa |
| Observed molecular weight | 35-37 kDa, 32-34 kDa |
| GenBank accession number | BC022286 |
| Gene symbol | AQP4 |
| Gene ID (NCBI) | 361 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
| IHC protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
| IF protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
| IP protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Early B cell tolerance defects in neuromyelitis optica favour anti-AQP4 autoantibody production. | ||
J Neuroinflammation Deficiency of TREK-1 potassium channel exacerbates blood-brain barrier damage and neuroinflammation after intracerebral hemorrhage in mice. | ||
Biochim Biophys Acta NADPH oxidase activation and 4-hydroxy-2-nonenal/aquaporin-4 adducts as possible new players in oxidative neuronal damage presents in drug-resistant epilepsy. | ||
Afr J Tradit Complement Altern Med PROTECTIVE EFFECTS OF ETHYL ACETATE EXTRACTION FROM GASTRODIA ELATA BLUME ON BLOOD-BRAIN BARRIER IN FOCAL CEREBRAL ISCHEMIA REPERFUSION. | ||
JCI Insight Attenuation of cerebral edema facilitates recovery of glymphatic system function after status epilepticus. |
