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Aquaporin 4 Antibody 20 Publications

Rabbit Polyclonal| Catalog number: 16473-1-AP

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

Aquaporin 4 Antibody WB mouse brain tissue 16473-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Aquaporin 4 Antibody IHC mouse brain tissue 16473-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Aquaporin 4 Antibody IHC mouse brain tissue 16473-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Aquaporin 4 Antibody WB mouse heart tissue 16473-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP(Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Aquaporin 4 Antibody IP mouse heart tissue 16473-1-AP

IP Result of anti-Aquaporin 4 (IP:16473-1-AP, 4ug; Detection:16473-1-AP 1:300) with mouse heart tissue lysate 4000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, SKOV-3 cells, mouse kidney tissue, mouse brain tissue
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
mouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:100-1:2500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Aquaporin 4 fusion protein Ag9561
Full name:
aquaporin 4
Calculated molecular weight:
323aa,35 kDa
Observed molecular weight:
35-37, 32-34 kDa
GenBank accession number:
BC022286
Gene ID (NCBI):
361
Gene symbol
AQP4
Synonyms
AQP 4, AQP4, aquaporin 4, NMO-IgG

Background

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.


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