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Aquaporin 4 Polyclonal antibody

Aquaporin 4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 16473-1-AP


AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, NMO-IgG, WCH4

Tested Applications

Positive WB detected inmouse liver tissue, SKOV-3 cells, mouse kidney tissue, mouse heart tissue, mouse brain tissue, rat brain tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, mouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:2500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16473-1-AP targets Aquaporin 4 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aquaporin 4 fusion protein Ag9561
Full Name aquaporin 4
Calculated Molecular Weight 323 aa, 35 kDa
Observed Molecular Weight 35-37 kDa, 32-34 kDa
GenBank Accession NumberBC022286
Gene Symbol AQP4
Gene ID (NCBI) 361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.


Product Specific Protocols
WB protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
IHC protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
IF protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
IP protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Reyes (Verified Customer) (04-05-2024)

Aquaporin 4 (in green) shows a strong stainning, although difficult for the analyses, in human FFPE brain cortex.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
Aquaporin 4 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:16473-1-AP)