• Featured Product
  • KD/KO Validated

Aquaporin 4 Antibody

Aquaporin 4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16473-1-AP

Synonyms

AQP 4, AQP4, aquaporin 4, NMO-IgG



Tested Applications

Positive WB detected inmouse heart tissue, SKOV-3 cells, mouse kidney tissue, mouse brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:2500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16473-1-AP is Aquaporin 4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aquaporin 4 fusion protein Ag9561
Full Name aquaporin 4
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight 35-37 kDa, 32-34 kDa
GenBank accession numberBC022286
Gene symbol AQP4
Gene ID (NCBI) 361
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
IHC protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
IP protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Brain

Early B cell tolerance defects in neuromyelitis optica favour anti-AQP4 autoantibody production.

Authors - Elizabeth Cotzomi
mouseWB

J Neuroinflammation

Deficiency of TREK-1 potassium channel exacerbates blood-brain barrier damage and neuroinflammation after intracerebral hemorrhage in mice.

Authors - Yongkang Fang
humanWB, IHC, IP

Biochim Biophys Acta

NADPH oxidase activation and 4-hydroxy-2-nonenal/aquaporin-4 adducts as possible new players in oxidative neuronal damage presents in drug-resistant epilepsy.

Authors - A Pecorelli
ratWB

Afr J Tradit Complement Altern Med

PROTECTIVE EFFECTS OF ETHYL ACETATE EXTRACTION FROM GASTRODIA ELATA BLUME ON BLOOD-BRAIN BARRIER IN FOCAL CEREBRAL ISCHEMIA REPERFUSION.

Authors - Fangyan He
humanWB

Exp Ther Med

Role of vasodilator-stimulated phosphoprotein in human cytomegalovirus-induced hyperpermeability of human endothelial cells.

Authors - Yihao Tian
ratWB,IHC,IF

Med Sci Monit

Aquaporin 4 Silencing Aggravates Hydrocephalus Induced by Injection of Autologous Blood in Rats.

Authors - Jian Guo
  • KD Validated