- Featured Product
- KD/KO Validated
Aquaporin 4 Polyclonal antibody
Aquaporin 4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 16473-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, SKOV-3 cells, mouse kidney tissue, mouse brain tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | mouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | rat brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:2500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 29 publications below |
IHC | See 14 publications below |
IF | See 16 publications below |
IP | See 1 publications below |
FC | See 1 publications below |
Product Information
The immunogen of 16473-1-AP is Aquaporin 4 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Aquaporin 4 fusion protein Ag9561 |
Full Name | aquaporin 4 |
Calculated molecular weight | 323 aa, 35 kDa |
Observed molecular weight | 35-37 kDa, 32-34 kDa |
GenBank accession number | BC022286 |
Gene symbol | AQP4 |
Gene ID (NCBI) | 361 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
IHC protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
IF protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
IP protocol for Aquaporin 4 antibody 16473-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Brain Early B cell tolerance defects in neuromyelitis optica favour anti-AQP4 autoantibody production. | ||
J Neuroinflammation Deficiency of TREK-1 potassium channel exacerbates blood-brain barrier damage and neuroinflammation after intracerebral hemorrhage in mice. | ||
Biochim Biophys Acta NADPH oxidase activation and 4-hydroxy-2-nonenal/aquaporin-4 adducts as possible new players in oxidative neuronal damage presents in drug-resistant epilepsy. | ||
Afr J Tradit Complement Altern Med PROTECTIVE EFFECTS OF ETHYL ACETATE EXTRACTION FROM GASTRODIA ELATA BLUME ON BLOOD-BRAIN BARRIER IN FOCAL CEREBRAL ISCHEMIA REPERFUSION. | ||
JCI Insight Attenuation of cerebral edema facilitates recovery of glymphatic system function after status epilepticus. |