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Aquaporin 4 Polyclonal antibody

Aquaporin 4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16473-1-AP

Synonyms

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, NMO-IgG, WCH4



Tested Applications

Positive WB detected inmouse heart tissue, SKOV-3 cells, mouse kidney tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:2500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16473-1-AP targets Aquaporin 4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aquaporin 4 fusion protein Ag9561
Full Name aquaporin 4
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight 35-37 kDa, 32-34 kDa
GenBank accession numberBC022286
Gene symbol AQP4
Gene ID (NCBI) 361
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
IHC protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
IF protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
IP protocol for Aquaporin 4 antibody 16473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Brain

Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia.

Authors - Aiqing Chen
mouseWB,IF

Brain Behav Immun

Polyunsaturated fatty acid supplement alleviates depression-incident cognitive dysfunction by protecting the cerebrovascular and glymphatic systems.

Authors - Xinghua Liu
mouseWB

Glia

Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice.

Authors - Wenqiang Quan
mouseWB

Brain Behav Immun

Hypoxia augments LPS-induced inflammation and triggers high altitude cerebral edema in mice.

Authors - Yanzhao Zhou
mouseIF

J Neuroinflammation

Regulatory T cells protect against brain damage by alleviating inflammatory response in neuromyelitis optica spectrum disorder.

Authors - Xue Ma
ratWB

J Neuroinflammation

Cottonseed oil alleviates ischemic stroke injury by inhibiting the inflammatory activation of microglia and astrocyte.

Authors - Min Liu