Aquaporin 4 Polyclonal antibody

• Featured Product
• KD/KO Validated

Aquaporin 4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

55

Cat no : 16473-1-AP

Synonyms

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, NMO-IgG, WCH4

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Validation Data Gallery

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  WB analysis using 16473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16473-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 16473-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16473-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16473-1-AP

  IP Result of anti-Aquaporin 4 (IP:16473-1-AP, 4ug; Detection:16473-1-AP 1:300) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of rat brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human kidney using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of rat brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red).

• 

  IF Staining of rat brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, SKOV-3 cells, mouse kidney tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:2500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 35 publications below
• IHC: See 15 publications below
• IF: See 18 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

16473-1-AP targets Aquaporin 4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aquaporin 4 fusion protein Ag9561
• Full Name: aquaporin 4
• Calculated molecular weight: 323 aa, 35 kDa
• Observed molecular weight: 35-37 kDa, 32-34 kDa
• GenBank accession number: BC022286
• Gene symbol: AQP4
• Gene ID (NCBI): 361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Publications

Species	Application	Title
human	IF	Brain; Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia.; Authors - Aiqing Chen
mouse	WB,IF	Brain Behav Immun; Polyunsaturated fatty acid supplement alleviates depression-incident cognitive dysfunction by protecting the cerebrovascular and glymphatic systems.; Authors - Xinghua Liu
mouse	WB	Glia; Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice.; Authors - Wenqiang Quan
mouse	WB	Brain Behav Immun; Hypoxia augments LPS-induced inflammation and triggers high altitude cerebral edema in mice.; Authors - Yanzhao Zhou
mouse	IF	J Neuroinflammation; Regulatory T cells protect against brain damage by alleviating inflammatory response in neuromyelitis optica spectrum disorder.; Authors - Xue Ma
rat	WB	J Neuroinflammation; Cottonseed oil alleviates ischemic stroke injury by inhibiting the inflammatory activation of microglia and astrocyte.; Authors - Min Liu

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