Aquaporin 4 Polyclonal antibody

• Featured Product
• KD/KO Validated

Aquaporin 4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC

Conjugate

Unconjugated

Publications

41

Cat no : 16473-1-AP

Synonyms

AQP 4, AQP4, aquaporin 4, NMO-IgG

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Validation Data Gallery

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  WB analysis using 16473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  WB analysis of mouse heart using 16473-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IP experiment of mouse heart using 16473-1-AP

  IP Result of anti-Aquaporin 4 (IP:16473-1-AP, 4ug; Detection:16473-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse heart tissue, SKOV-3 cells, mouse kidney tissue, mouse brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue, rat brain tissue, mouse heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: rat brain tissue

Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:2500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 13 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

The immunogen of 16473-1-AP is Aquaporin 4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aquaporin 4 fusion protein Ag9561
• Full Name: aquaporin 4
• Calculated molecular weight: 323 aa, 35 kDa
• Observed molecular weight: 35-37 kDa, 32-34 kDa
• GenBank accession number: BC022286
• Gene symbol: AQP4
• Gene ID (NCBI): 361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Publications

Species	Application	Title
		Brain; Early B cell tolerance defects in neuromyelitis optica favour anti-AQP4 autoantibody production.; Authors - Elizabeth Cotzomi
mouse	WB	J Neuroinflammation; Deficiency of TREK-1 potassium channel exacerbates blood-brain barrier damage and neuroinflammation after intracerebral hemorrhage in mice.; Authors - Yongkang Fang
human	WB, IHC, IP	Biochim Biophys Acta; NADPH oxidase activation and 4-hydroxy-2-nonenal/aquaporin-4 adducts as possible new players in oxidative neuronal damage presents in drug-resistant epilepsy.; Authors - A Pecorelli
rat	WB	Afr J Tradit Complement Altern Med; PROTECTIVE EFFECTS OF ETHYL ACETATE EXTRACTION FROM GASTRODIA ELATA BLUME ON BLOOD-BRAIN BARRIER IN FOCAL CEREBRAL ISCHEMIA REPERFUSION.; Authors - Fangyan He
human	IF	bioRxiv; Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.; Authors - Madeline G Andrews
mouse	IF	JCI Insight; Attenuation of cerebral edema facilitates recovery of glymphatic system function after status epilepticus.; Authors - Kewei Liu

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