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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Aquaporin 4 Polyclonal antibody

Aquaporin 4 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16473

Synonyms

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, WCH4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Aquaporin 4 Polyclon (CL488-16473)

    IF Staining of mouse brain using CL488-16473

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Aquaporin 4 antibody (CL488-16473) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-16473 is Aquaporin 4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aquaporin 4 fusion protein Ag9561
Full Name aquaporin 4
Calculated Molecular Weight 323 aa, 35 kDa
Observed Molecular Weight 35-37 kDa, 32-34 kDa
GenBank Accession NumberBC022286
Gene Symbol AQP4
Gene ID (NCBI) 361
RRIDAB_2919135
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Aquaporin 4 antibody CL488-16473Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tinne (Verified Customer) (06-27-2023)

I found this antibody to work really cell in IF!

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mixed culture of human neurons and astrocytes
CL Plus 488 Aquaporin 4 Antibody Immunofluorescence validation (1:500 dilution) in Mixed culture of human neurons and astrocytes (Cat no:CL488-16473)