AQP4 Monoclonal antibody

AQP4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G7D3

Cat no : 68448-1-Ig

Synonyms

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, WCH4

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Validation Data Gallery

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  WB analysis using 68448-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spinal cord using 68448-1-Ig

  rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: pig brain tissue, rat spinal cord tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68448-1-Ig targets AQP4 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: AQP4 fusion protein Ag9830
• Full Name: aquaporin 4
• Calculated Molecular Weight: 323 aa, 35 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC022286
• Gene Symbol: AQP4
• Gene ID (NCBI): 361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.