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  • KD/KO Validated

Amphiregulin Polyclonal antibody

Amphiregulin Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 16036-1-AP

Synonyms

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF



Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16036-1-AP targets Amphiregulin in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Amphiregulin fusion protein Ag8907
Full Name amphiregulin
Calculated molecular weight 252 aa, 28 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC009799
Gene symbol AREG
Gene ID (NCBI) 374
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

Protocols

Product Specific Protocols
WB protocol for Amphiregulin antibody 16036-1-APDownload protocol
IHC protocol for Amphiregulin antibody 16036-1-APDownload protocol
IF protocol for Amphiregulin antibody 16036-1-APDownload protocol
IP protocol for Amphiregulin antibody 16036-1-APDownload protocol
FC protocol for Amphiregulin antibody 16036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

CD317 Activates EGFR by Regulating Its Association with Lipid Rafts.

Authors - Guizhong Zhang
humanIP

Proc Natl Acad Sci U S A

AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer.

Authors - Shawn P Kubli
humanWB, IF

Oncogene

The EGF receptor ligand amphiregulin controls cell division via FoxM1.

Authors - S W Stoll
humanWB,IHC,IP

Aging Cell

Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.

Authors - Qixia Xu
  • KD Validated
humanWB,IF

J Invest Dermatol

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.

Authors - Stefan W Stoll
humanIHC

Cell Cycle

TAZ induces growth factor-independent proliferation through activation of EGFR ligand amphiregulin.

Authors - Yang Nuo N