ARHGEF1 Polyclonal antibody

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  WB analysis of HEK-293 using 11363-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11363-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11363-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11363-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11363-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11363-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11363-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11363-1-AP

  IP result of anti-ARHGEF1 (IP:11363-1-AP, 4ug; Detection:11363-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon using 11363-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 11363-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 11363-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARHGEF1 antibody (11363-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 11363-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ARHGEF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11363-1-AP

Publications(5)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

GEF1, LBCL2, LSC, P115 RHOGEF, p115RhoGEF

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, human spleen tissue, Jurkat cells, K-562 cells, mouse spleen tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• CoIP: See 1 publications below

Product Information

11363-1-AP targets ARHGEF1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1905

  Product name: Recombinant human ARHGEF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC011726

  Sequence: MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEP

• Full Name: Rho guanine nucleotide exchange factor (GEF) 1
• Calculated Molecular Weight: 912 aa, 102 kDa
• Observed Molecular Weight: 102-110 kDa
• GenBank Accession Number: BC011726
• Gene Symbol: ARHGEF1
• Gene ID (NCBI): 9138
• RRID: AB_2059741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92888
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF1, also known as p115-RhoGEF, is a member of the Rho guanine nucleotide exchange factor (GEF) family, specifically activating the small GTPase RhoA by catalyzing the exchange of GDP for GTP. Structurally, it contains a Dbl homology (DH) domain responsible for GEF activity, a Pleckstrin homology (PH) domain that may regulate membrane association, and an RGS-like domain that interacts with the G-protein subunits Gα12/13. This protein is expressed ubiquitously, with particularly high expression in hematopoietic and lymphoid tissues, and plays a critical role in transducing signals from G protein-coupled receptors coupled to Gα12/13 to activate RhoA and its downstream effector ROCK, thereby regulating cytoskeletal dynamics, cell contraction, and migration. Notably, ARHGEF1 acts as a dual-specificity regulator, functioning as a GAP for Gα12/13 while serving as a GEF for RhoA. Its involvement in essential physiological processes, such as angiotensin II-induced vascular smooth muscle contraction, underscores its importance, while mutations in the ARHGEF1 gene are associated with immunodeficiency 62 and other potential pathologies.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.; Authors - Silvana Konermann
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Mol Biol Cell; The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.; Authors - P V Ryder
human	WB	Cancer Lett; Apigenin inhibits tumor angiogenesis by hindering microvesicle biogenesis via ARHGEF1; Authors - Wanying Zhang
mouse	WB,CoIP	Nat Commun; Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis; Authors - Yaping Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (12-29-2023)

Worked well for IP using 6ug Ab and WB 1:1500

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioma

FH

can (Verified Customer) (12-17-2019)

Very good antibody!

• Applications: Immunoprecipitation,
• Cell Tissue Type: blood vascular cell

FH

Ni (Verified Customer) (09-26-2019)

Very successful in immunoprecipitating p115RhoGEF from transfected cells.

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 2 ug /reaction