ARPC1B Monoclonal antibody

ARPC1B Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C8A1

Cat no : 67320-1-Ig

Synonyms

ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC

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Validation Data Gallery

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  WB analysis using 67320-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67320-1-Ig (ARPC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 67320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 67320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 67320-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, THP-1 cells, rat spleen tissues
• Positive IHC detected in: human appendicitis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67320-1-Ig targets ARPC1B in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARPC1B fusion protein Ag29095
• Full Name: actin related protein 2/3 complex, subunit 1B, 41kDa
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC007555
• Gene Symbol: ARPC1B
• Gene ID (NCBI): 10095
• RRID: AB_2882580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARPC1B also known as ARC41, p41-ARC, or p40-ARC, is one of seven subunits of the human Arp2/3 protein complex, that belongs to the SOP2 family. ARPC1B localizes on centrosomes and has a distinct role in centrosomal homeostasis. ARPC1B is both a physiological activator and substrate of Aurora A kinase, and these interactions help to maintain mitotic integrity in mammalian cells. In recent resaeach, ARPC1B is selected as a candidate prediction marker for sensitivity of Choroidal malignant melanomas to radiotherapy.