Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse thymus tissue |
| Positive IHC detected in | mouse kidney tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16717-1-AP targets ARPC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10144 Product name: Recombinant human ARPC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC057237 Sequence: MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRHSITGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV Predict reactive species |
| Full Name | actin related protein 2/3 complex, subunit 5, 16kDa |
| Calculated Molecular Weight | 154 aa, 17 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | BC057237 |
| Gene Symbol | ARPC5 |
| Gene ID (NCBI) | 10092 |
| RRID | AB_1850902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15511 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARPC5 antibody 16717-1-AP | Download protocol |
| IHC protocol for ARPC5 antibody 16717-1-AP | Download protocol |
| WB protocol for ARPC5 antibody 16717-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARPC5 Polyclonal antibody (Cat# 16717-1-AP) has 7 citations published in journals including Advanced Science, International Journal of Molecular Sciences, Journal of Neuroscience, BMC Cancer, Heliyon, Pigment Cell & Melanoma Research, and Journal of Reproduction and Development. Research fields span actin dynamics and viral trafficking, oxidative stress and liver injury, synapse biology and schizophrenia, glioma prognosis, endothelial senescence in diabetes, melanoma migration, and steroidogenesis in reproductive cells.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Adducin-1 Facilitates Influenza Virus Endosomal Trafficking and Uncoating by Regulating Branched Actin Dynamics and Myosin IIB Activity | ||
Int J Mol Sci Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis | ||
J Neurosci The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse. | ||
BMC Cancer ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas | ||
Heliyon Dapagliflozin impedes endothelial cell senescence by activating the SIRT1 signaling pathway in type 2 diabetes | ||
Pigment Cell Melanoma Res YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5) |
