Tested Applications
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-16717 targets ARPC5 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10144 Product name: Recombinant human ARPC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC057237 Sequence: MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRHSITGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV Predict reactive species |
| Full Name | actin related protein 2/3 complex, subunit 5, 16kDa |
| Calculated Molecular Weight | 154 aa, 17 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | BC057237 |
| Gene Symbol | ARPC5 |
| Gene ID (NCBI) | 10092 |
| RRID | AB_3083989 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15511 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARPC5 antibody CL488-16717 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |

